CRISPR-based modular assembly of a UAS-cDNA/ORF plasmid library for more than 5500 Drosophila genes conserved in humans
نویسندگان
چکیده
منابع مشابه
A versatile platform for creating a comprehensive UAS-ORFeome library in Drosophila.
Overexpression screens are used to explore gene functions in Drosophila, but this strategy suffers from the lack of comprehensive and systematic fly strain collections and efficient methods for generating such collections. Here, we present a strategy that could be used efficiently to generate large numbers of transgenic Drosophila strains, and a collection of 1149 UAS-ORF fly lines that were cr...
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ژورنال
عنوان ژورنال: Genome Research
سال: 2019
ISSN: 1088-9051,1549-5469
DOI: 10.1101/gr.250811.119